Cul4B Antibody

• Catalog Number: orb1524036
• Category: Antibodies
• Description: Cul4B Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, WB
• Reactivity: Human, Mouse
• Immunogen: Between 863 and 913
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Cul4B
• UniProt ID: Q13620
• NCBI: NP_003579.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: CUL-4B; SFM2; MRXSC; cullin-4B; MRXS15; MRXHF2
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Cul4B by western blot. Samples: Whole cell lysate from HEK293T (15 and 50 µg) , HeLa (50µg) , and Jurkat (50µg) cells. Antibodies: Affinity purified rabbit anti-Cul4B antibody orb1524036 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of mouse Cul4B by immunohistochemistry.Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Cul4B (Cat. No. orb1524036) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Cul4B by immunohistochemistry.Sample: FFPE section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-Cul4B (Cat. No. orb1524036) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).