CUG-BP1/2 Rabbit Polyclonal Antibody

SKU: orb182891

Description

CUG-BP1/2 Rabbit Polyclonal Antibody

Research Area

DNA / RNA Binding, DNA and RNA Research, RNA Processing

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P
• Dilution Range: IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Mouse, Porcine, Rabbit, Xenopus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CUG-BP1 and CUG-BP2 (421-508/508aa)
• Target: CELF2
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CELF2_CHICK; CELF2; CELF-2; Bruno-like protein 3; CUG triplet repeat RNA-binding protein 2 (CUG-BP2); CUG-BP- and ETR-3-like factor 2; ELAV-type RNA-binding protein 3 (ETR-3); RNA-binding protein BRUNOL-3; CUGBP2; CELF2_HUMAN; Neuroblastoma apoptosis-related RNA-binding protein (hNAPOR); BRUNOL3; ETR3; NAPOR; CELF2_MOUSE; ELAV-type RNA-binding protein 3 (ETR-3 | mETR-3); Neuroblastoma apoptosis-related RNA-binding protein (mNapor); CELF2_XENLA; CELF2_DANRE;

Paraformaldehyde-fixed, paraffin embedded (rat brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CUG-BP1, 2) Polyclonal Antibody, Unconjugated (orb182891) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat heart), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CUG-BP1, 2) Polyclonal Antibody, Unconjugated (orb182891) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (rat lung), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CUG-BP1, 2) Polyclonal Antibody, Unconjugated (orb182891) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Tissue/Cell: human lung carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CUG-BP1/2 Polyclonal Antibody, Unconjugated (orb182891) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

CELF2

UniProt

O95319