CTRP3 Antibody

• Catalog Number: orb1239313
• Category: Antibodies
• Description: CTRP3 Antibody
• Target: C1QTNF3
• Clonality: Polyclonal
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: 1 mg/mL
• Buffer/Preservatives: CTRP3 Antibody is supplied in PBS containing 0.02% sodium azide.
• Purification: CTRP3 Antibody is affinity chromatography purified via peptide column.
• Immunogen: Anti-CTRP3 antibody (orb1239313) was raised against a peptide corresponding to 16 amino acids near the center of human CTRP3. The immunogen is located within amino acids 90-140 of CTRP3.
• UniProt ID: Q9BXJ4
• MW: Predicted: 27kDObserved: 30 kD (Post-modyfication: 1 N-linked glycosylation)
• Tested applications: ELISA, IF, IHC-P, WB
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CTRP3 Antibody: CORS, CORCS, CTRP3, CORS26, C1ATNF
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_852100

Western Blot Validation in Human and Mouse Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation with Recombinant Protein. Loading: 30 ng of human CTRP3 recombinant protein per lane. Antibodies: CTRP3, orb1239313 (2 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse and Rat Tissue Lysates. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot Validation in Mouse Heart Tissue Lysate in the (A) absence and (B) presence of blocking peptide. Loading: 15 µg of lysates per lane. Antibodies: CTRP3, orb1239313 (1 µg/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Immunohistochemistry Validation of CTRP3 in Mouse Heart Tissue. Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-CTRP3 antibody (orb1239313) at 2 µg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.

Immunofluorescence Validation of CTRP3 in Mouse Heart Tissue. Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling CTRP3 with orb1239313 at 10 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).