CTLA4 antibody

• Catalog Number: orb758791
• Category: Antibodies
• Description: Rabbit monoclonal antibody to CTLA4
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 9D9
• Tested applications: Blocking, IF
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: The antibody was raised against CTLA-4.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: CTLA-4
• UniProt ID: P09793
• Storage: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD152; CTLA4; Cytotoxic T-Lymphocyte Associated Pr
• Note: For research use only
• Application notes: The antibody has been used to block CTLA-4 and has been shown to enhance T-cell immunity against tumours (Quezada et al, 2006). 9D9 also has an anti-B16/BL6 melanoma effect in non-irradiated tumour-bearing recipients when given as combinatorial therapy with Gvax (Peggs et al, 2009). Selby et al. (PMID: 24777248) show how conversion of the original mouse IgG2b to a mouse IgG2a greatly increases anti-tumor activity of the 9D9 antibody in both MC38 (C57BL/6 mice) and CT26 (BALB/c mice) models. This mechanism both involves blockade of CTLA4 as well as depletion of tumor-infiltrating regulatory T-cells. Fc Silent antibodies were shown to have reduced anti-tumor activity as they did not deplete tumor infiltrating regulatory T-cells, however they lead to expansion of peripheral Tregs.
• Expiration Date: 12 months from date of receipt.