CTLA4 Antibody

• Catalog Number: orb1239311
• Category: Antibodies
• Description: CTLA4 Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2G10
• Tested applications: ELISA, ICC, IF, IHC-P, WB
• Reactivity: Human
• Isotype: IgG1
• Immunogen: CTLA-4 antibody was raised against the extracellular domain of human CTLA-4.
• Concentration: 1 mg/mL
• Dilution range: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 25 kDa Observed: 32 kDa
• Target: CTLA4
• UniProt ID: P16410
• NCBI: NP_005205
• Storage: CTLA-4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: CTLA-4 Antibody is supplied in PBS containing 0.02% sodium azide and 50% glycerol.
• Alternative names: CTLA-4 Antibody: CTL4, cytotoxic T-lymphocyte asso
• Note: For research use only
• Application notes: CTLA-4 antibody can be used for detection of CTLA-4 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.5 and 1 μg/mL.

Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.

Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [2G10] (orb1239311) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [2G10] (orb1239311) Blue: DAPI staining

Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL. Red: CTLA4 Antibody [2G10] (orb1239311) Blue: DAPI staining

Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.

Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.

Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with orb1239307, orb1239311, and orb1239312 antibodies at decreasing concentrations.