CTLA4 antibody

• Catalog Number: orb222748
• Category: Antibodies
• Description: Mouse monoclonal antibody to CTLA4
• Clonality: Monoclonal
• Clone Number: BNI3
• Tested applications: FC, ICC, IHC-Fr, IP
• Reactivity: Human
• Isotype: Mouse IgG2a
• Immunogen: Human CD152-IgG heavy chain fusion protein
• Concentration: 1 mg/ml
• Dilution range: Flow cytometry: Recommended dilution: 2-5 μg/ml, Intracellular staining.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: CD152
• Entrez: 1493
• UniProt ID: P16410
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti-ALPS5 antibody, anti-CD 152 antibody, anti-CD
• Note: For research use only
• Application notes: Flow cytometry: Recommended dilution: 2-5 μg/ml, Intracellular staining.
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human PHA stimulated lymphocytes stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml, GAM APC) and anti-human CD3 (UCHT1) PE antibody (20 μl reagent / 100 μl of peripheral whole blood).

Separation of human CD152 positive CD3 positive lymphocytes (red-filled) from CD152 negative CD3 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml) GAM APC.

Flow cytometry surface staining pattern of human PHA stimulated peripheral whole blood stained using anti-human CD152 (BNI3) purified antibody (concentration in sample 10 µg/ml) GAM APC.