CTCF Antibody

• Catalog Number: orb308788
• Category: Antibodies
• Description: CTCF Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Predicted Reactivity: Bovine, Monkey, Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived human CTCF recombinant protein (Position: K521-R727). Human CTCF shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat CTCF, respectively.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 82785 MW
• UniProt ID: P49711
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Transcriptional repressor CTCF;11-zinc finger prot
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

WB analysis of CTCF using anti-CTCF antibody.Lane 1:human 293T cell;2:human Caco-2 cell;3:rat C6 cell;4:mouse brain tissue;5:mouse RAW264.7 cell.

IF analysis of CTCF using anti-CTCF antibody. CTCF was detected in an immunocytochemical section of U20S cells.

IHC analysis of CTCF using anti-CTCF antibody. CTCF was detected in a paraffin-embedded section of Human Lung Cancer tissue.

IHC analysis of CTCF using anti-CTCF antibody. CTCF was detected in a paraffin-embedded section of Rat Intestine tissue.

IHC analysis of CTCF using anti-CTCF antibody. CTCF was detected in a paraffin-embedded section of Mouse Intestine tissue.