CTCF Antibody

• Catalog Number: orb1519896
• Category: Antibodies
• Description: CTCF Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IHC, IP, SW-Size, WB
• Reactivity: Human, Mouse
• Immunogen: between 650 and 700
• Concentration: 100 µg/ml
• Dilution range: WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: CTCF
• UniProt ID: P49711
• NCBI: NP_006556.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: CTCFL paralog; MRD21; CCCTC-binding factor (zinc f
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse CTCF by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CTCF by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, MCF-7, Jurkat (10 µg) , Hep-G2, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human CTCF by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse CTCF by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTCF by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CTCF recombinant monoclonal antibody [BL-395-3B3] (orb1519896) used for IP at 20 µl/mg lysate.

Detection of mouse CTCF by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human CTCF by immunocytochemistry. Sample: FFPE section of MCF-7 cells. Antibody: Rabbit anti-CTCF recombinant monoclonal antibody (orb1519896). Secondary: HRP-conjugated goat anti-rabbit IgG.