CtBP1 Antibody

• Catalog Number: orb259602
• Category: Antibodies
• Description: CtBP1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CtBP1 (409-440aa HGLPPVAHPPHAPSPGQTVKPEADRDHASDQL), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse, Rat, By Heat Immunocytochemistry/Immunofluorescence, 2μg/ml, Human Immunofluorescence, 2μg/ml, Human, Rat Flow Cytometry, 1-3μg/1x106 cells, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 47535 MW
• UniProt ID: Q13363
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: C-terminal-binding protein 1;CtBP1;1.1.1.-;CTBP1;C
• Note: For research use only
• Application notes: Tested Species: In-house tested species with positive results. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

Flow Cytometry analysis of U20S cells using anti-CTBP1 antibody (Blue line).Isotype control antibody (Green line) was rabbit IgG .Unlabelled sample (Red line) was also used as a control.

WB analysis of CTBP1 using anti-CTBP1 antibody.Lane 1:human SH-SY5Y cell;2:human MOLT-4 cell;3:human MCF-7 cell;4:rat PC-12 cell;5:mouse SP2/0 cell.

IF analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of human intestinal cancer tissues.

IF analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of rat intestine tissues.

IF analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in immunocytochemical section of U20S cell.

IF analysis of CTBP1 using anti-CTBP1 antibody and anti-Tubulin Alpha antibody.CTBP1 was detected in immunocytochemical section of T47D cell.

IHC analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of Mouse Thymus Tissue.

IHC analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of Rat Spleen Tissue.

IHC analysis of CTBP1 using anti-CTBP1 antibody.CTBP1 was detected in paraffin-embedded section of Human Tonsil Tissue.