CSF1 Rabbit Polyclonal Antibody

• Catalog Number: orb325141
• Category: Antibodies
• Description: Rabbit polyclonal antibody to CSF1
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC-P, WB
• Predicted Reactivity: Canine, Guinea pig, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen is a synthetic peptide directed towards the middle region of human CSF1
• Concentration: Batch dependent within range: 100 μl at 0.5-1 mg/ml
• Form/Appearance: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Conjugation: Unconjugated
• MW: 61 kDa
• Target: CSF1
• UniProt ID: P09603
• Protein Sequence: Synthetic peptide located within the following region: SGSVLPLGELEGRRSTRDRRSPAEPEGGPASEGAARPLPRFNSVPLTDTG
• NCBI: NP_000748
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Alternative names: anti MCSF antibody, anti MGC31930 antibody, anti C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

25 ug of the indicated Human whole cell extracts was loaded onto a 12% SDS-PAGE gel. 1 ug/mL of the antibody was used in this experiment. Peptide is cross-reacting with CSF4 at ~59 kDa, and is also present in isoforms of CSF1 at ~56 kDa and ~51 kDa.

CSF1 in human placenta was detected using HRP/AEC red color stain, Working dilution 10 ug/mL.

Sample Tissue: Human Fetal Liver, Antibody Dilution: 1.0 ug/mL.

Sample Tissue: Human Jurkat Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Tissue: Human Liver Tumor, Antibody Dilution: 1 ug/mL.

Sample Tissue: Human THP-1 Whole Cell, Antibody Dilution: 1 ug/mL.

Sample Type: 721_B, Antibody Dilution: 1.0 ug/mL, CSF1 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells.

Positive control (+): Human liver (LI), Negative control (-): MCF7 (N10), Antibody concentration: 3 ug/mL.

WB Suggested Anti-CSF1 Antibody Titration: 0.2-1 ug/mL, ELISA Titer: 1:62500, Positive Control: COLO205 cell lysate.

WB Suggested Anti-CSF1 antibody Titration: 1 ug/mL, Sample Type: Human heart.