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Catalog Number | orb517017 |
---|---|
Category | Antibodies |
Description | Crotonyl-HIST1H2BC (K16) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ChIP, ELISA, ICC, IF, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Peptide sequence around site of Crotonyl-Lys (16) derived from Human Histone H2B type 1-C/E/F/G/I |
Dilution range | WB:1:500-1:5000, ICC:1:500-1:1000, IF:1:50-1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | HIST1H2BC |
UniProt ID | P62807 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
Alternative names | H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibod Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Crotonyl-HIST1H2BC (K16) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using re
Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysateusing Crotonyl-HIST1H2BC (K16) antibody
Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) using Crotonyl-HIST1H2BC (K16) antibody
Immunocytochemistry analysis of Hela cells using Crotonyl-HIST1H2BC (K16) antibody