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Crotonyl-HIST1H2BC (K16) Antibody
Description
Images & Validation
−| Tested Applications | ChIP, ELISA, ICC, IF, WB |
|---|---|
| Dilution range | WB:1:500-1:5000, ICC:1:500-1:1000, IF:1:50-1:200 |
| Reactivity | Human |
Key Properties
−| Host | Rabbit |
|---|---|
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Peptide sequence around site of Crotonyl-Lys (16) derived from Human Histone H2B type 1-C/E/F/G/I |
| Target | HIST1H2BC |
| Purification | Antigen Affinity Purified |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol |
| Disclaimer | For research use only |
Alternative Names
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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Crotonyl-HIST1H2BC (K16) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using re

Western blot aanalysis of Hela whole cell lysate, 293 whole cell lysate, A549 whole cell lysate, K562 whole cell lysateusing Crotonyl-HIST1H2BC (K16) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) using Crotonyl-HIST1H2BC (K16) antibody

Immunocytochemistry analysis of Hela cells using Crotonyl-HIST1H2BC (K16) antibody
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UniProt Details
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Protocol Information
Crotonyl-HIST1H2BC (K16) Antibody (orb517017)
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