Crotonyl-HIST1H2BC (K12) Antibody

• Catalog Number: orb516361
• Category: Antibodies
• Description: Crotonyl-HIST1H2BC (K12) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, ELISA, IF, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I
• Dilution range: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: HIST1H2BC
• UniProt ID: P62807
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Alternative names: H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibod
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Crotonyl-HIST1H2BC (K12) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using re

Western blot aanalysis of Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysateusing Crotonyl-HIST1H2BC (K12) antibody

Immunoprecipitation analysis of 293 whole cell lysate using Crotonyl-HIST1H2BC (K12) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) using Crotonyl-HIST1H2BC (K12) antibody