HIST1H2BC Antibody

SKU: orb516361

Description

This Crotonyl-HIST1H2BC (K12) Antibody is an unconjugated polyclonal product. It tagets HIST1H2BC using a Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IP, WB. Purification: Antigen Affinity Purified.

Images & Validation

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Tested Applications	ChIP, ELISA, IF, IP, WB
Dilution range	WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
Reactivity	Human

Key Properties

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Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I
Target	HIST1H2BC
Purification	Antigen Affinity Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
Disclaimer	For research use only

Alternative Names

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H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody

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Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Crotonyl-HIST1H2BC (K12) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using re

Western blot aanalysis of Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysateusing Crotonyl-HIST1H2BC (K12) antibody

Immunoprecipitation analysis of 293 whole cell lysate using Crotonyl-HIST1H2BC (K12) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) using Crotonyl-HIST1H2BC (K12) antibody

Quick Database Links

Gene Symbol

HIST1H2BC

UniProt

P62807

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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ChIP

Chromatin Immunoprecipitation

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