Crotonyl-HIST1H2BC (K12) Antibody

SKU: orb516361

Description

This Crotonyl-HIST1H2BC (K12) Antibody is an unconjugated polyclonal product. It tagets HIST1H2BC using a Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I as the immunogen. This antibody is suitable for ChIP, ELISA, IF, IP, WB. Purification: Antigen Affinity Purified.

Images & Validation

• Tested Applications: ChIP, ELISA, IF, IP, WB
• Dilution range: WB:1:500-1:5000, IF:1:50-1:200, IP:1:200-1:2000
• Reactivity: Human

Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Peptide sequence around site of Crotonyl-Lys (12) derived from Human Histone H2B type 1-C/E/F/G/I
• Target: HIST1H2BC
• Purification: Antigen Affinity Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.03% Proclin 300. Constituents: 50% Glycerol
• Disclaimer: For research use only

Alternative Names

H2BC4 antibody; H2BFL antibody; HIST1H2BC; antibody; H2BC6 antibody; H2BFH antibody; HIST1H2BE; antibody; H2BC7 antibody; H2BFG antibody; HIST1H2BF; antibody; H2BC8 antibody; H2BFA antibody; HIST1H2BG; antibody; H2BC10 antibody; H2BFK antibody; HIST1H2BIHistone H2B type 1-C/E/F/G/I antibody; Histone H2B.1 A antibody; Histone H2B.a antibody; H2B/a antibody; Histone H2B.g antibody; H2B/g antibody; Histone H2B.h antibody; H2B/h antibody; Histone H2B.k antibody; H2B/k antibody; Histone H2B.l antibody; H2B/l antibody

Chromatin Immunoprecipitation Hela (4*106) were treated with Micrococcal Nuclease, sonicated, and immunoprecipitated with 5ug anti- Crotonyl-HIST1H2BC (K12) antibody or a control normal rabbit IgG. The resulting ChIP DNA was quantified using re

Western blot aanalysis of Hela whole cell lysate, K562 whole cell lysate, 293 whole cell lysateusing Crotonyl-HIST1H2BC (K12) antibody

Immunoprecipitation analysis of 293 whole cell lysate using Crotonyl-HIST1H2BC (K12) antibody

Immunofluorescence staining of Hela cells (treated with 30mM sodium crotonylate for 4h) using Crotonyl-HIST1H2BC (K12) antibody

Quick Database Links

Gene Symbol

HIST1H2BC

UniProt

P62807