CROT Antibody

• Catalog Number: orb1262243
• Category: Antibodies
• Description: CROT Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This CROT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-79 amino acids from the N-terminal region of human CROT.
• Concentration: batch dependent
• Dilution range: For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 70 kDa
• Target: CROT
• UniProt ID: Q9UKG9
• NCBI: Q9UKG9
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Peroxisomal carnitine O-octanoyltransferase, COT,
• Note: For research use only
• Application notes: For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:50~100For WB starting dilution is: 1:1000
• Expiration Date: 12 months from date of receipt.

Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Formalin-fixed and paraffin-embedded human brain tissue reacted with CROT Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Western blot analysis of CROT Antibody in K562 cell line and mouse liver tissue lysates (35 ug/lane)