CRLS1 Antibody

• Catalog Number: orb1248831
• Category: Antibodies
• Description: CRLS1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-RSALGSALDPLADK
• Antibody Type: Primary Antibody
• Concentration: 500 ug/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Approx 30 kDa
• Target: CRLS1
• UniProt ID: Q9UJA2
• NCBI: NP_001120930.1, NP_061968.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: CRLS1, cardiolipin synthase 1, C20orf155, CLS, CLS
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 30kDa band observed in lysates of cell line HEK293 (calculated MW of 32.6kDa according to NP_061968.1). Recommended concentration: 0.2-0.6ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Kidney. Recommended concentration: 5ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1248831 (0.2 ug/ml) staining of HEK293 lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

orb1248831 (5 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.