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Catalog Number | orb2294141 |
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Category | Antibodies |
Description | Rabbit polyclonal antibody raised against a full-length human CRKL protein. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Immunogen | CRKL (NP_005198.1, 1 a.a. ~ 303 a.a) full-length human protein. |
Protein Sequence | MSSARFDSSDRSAWYMGPVSRQEAQTRLQGQRHGMFLVRDSSTCPGDYVLSVSENSRVSHYIINSLPNRRFKIGDQEFDHLPALLEFYKIHYLDTTTLIEPAPRYPSPPMGSVSAPNLPTAEDNLEYVRTLYDFPGNDAEDLPFKKGEILVIIEKPEEQWWSARNKDGRVGMIPVPYVEKLVRSSPHGKHGNRNSNSYGIPEPAHAYAQPQTTTPLPAVSGSPGAAITPLPSTQNGPVFAKAIQKRVPCAYDKTALALEVGDIVKVTRMNINGQWEGEVNGRKGLFPFTHVKIFDPQNPDENE |
Tested applications | IF, PLA, WB |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | NP_005198.1 |
CRKL MaxPab rabbit polyclonal antibody. Western Blot analysis of CRKL expression in A-431.
Immunofluorescence of purified MaxPab antibody to CRKL on HeLa cell. [antibody concentration 10 ug/ml].
Proximity Ligation Analysis of protein-protein interactions between CRKL and EGFR. HeLa cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-EGFR mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Analysis of protein-protein interactions between CRKL and PTPN11. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:600 and anti-PTPN11 mouse purified polyclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Analysis of protein-protein interactions between CRKL and SOS1. Huh7 cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL MaxPab polyclonal antibody. Lane 1: CRKL transfected lysate(33.80 KDa). Lane 2: Non-transfected lysate.