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Catalog Number | orb2294144 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody raised against a full-length human CRK protein. |
Clonality | Polyclonal |
Species/Host | Rabbit |
Conjugation | Unconjugated |
Reactivity | Human |
Buffer/Preservatives | In 1x PBS, pH 7.4 |
Immunogen | CRK (NP_005197.3, 1 a.a. ~ 204 a.a) full-length human protein. |
Protein Sequence | MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGR |
Tested applications | IF, PLA, WB |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Note | For research use only |
NCBI | NP_005197.3 |
Immunofluorescence of purified MaxPab antibody to CRK on HeLa cell. [antibody concentration 10 ug/ml].
Proximity Ligation Analysis of protein-protein interactions between CRK and SHC1. Mahlavu cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SHC1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Proximity Ligation Analysis of protein-protein interactions between CRK and SOS1. HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-SOS1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot analysis of CRK expression in transfected 293T cell line by CRK MaxPab polyclonal antibody. Lane 1: CRK transfected lysate(22.90 KDa). Lane 2: Non-transfected lysate.