CRISP2 Antibody

SKU: orb1249168

Description

CRISP2 Antibody

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Reactivity: Human
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:In transfected HEK293 transiently expressing Human CRISP2 (MYC and DYKDDDDK tagged), a band of approx. 35kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody. Recommended concentration: 0.5-1µg/mL. Primary incubations were for 1 hour. Preliminary experiments gave an approx 55kDa band in Human Testis lysates after 1µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 27.3kDa according to NP_003287.1. The 55kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75µg/mL.

Key Properties

• Antibody Type: Primary Antibody
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen for this antibody is: C-QHSDPEDRKTSTR
• Target: CRISP2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 500 ug/mL
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CRISP2, cysteine-rich secretory protein 2, CRISP-2, GAPDL5, MGC111136, TPX1, TSP1, OTTHUMP00000016589, glyceraldehyde-3-phosphate dehydrogenase-like 5, testis specific protein 1 (probe H4-1 p3-1)

HEK293 lysate (10 ug protein in RIPA buffer) overexpressing Human CRISP2 (RC205312) with C-terminal MYC tag probed with orb1249168 (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C.

orb1249168 (3.75 ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

CRISP2

UniProt

P16562

RefSeq (Protein):NP_003287.1