CREBBP monoclonal antibody (M01), clone 2H5

• Catalog Number: orb2294165
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a partial recombinant CREBBP.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2H5
• Tested applications: ELISA, IF, PLA, WB
• Reactivity: Human
• Isotype: IgG1 kappa
• Immunogen: CREBBP (NP_004371, 951 a.a. ~ 1050 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: PVHAQPPGTPLSQAAASIDNRVPTPSSVASAETNSQQPGPDVPVLEMKTETQAEDTEPDPGESKGEPRSEMMEEDLQGASQVKEETDIAEQKSEPMEVDE
• NCBI: NP_004371
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged CREBBP is approximately 0.03 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to CREBBP on HeLa cell. [antibody concentration 10 ug/ml].

Proximity Ligation Analysis of protein-protein interactions between FGFR1 and CREBBP. HeLa cells were stained with anti-FGFR1 rabbit purified polyclonal 1:1200 and anti-CREBBP mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Western Blot detection against Immunogen (36.74 KDa).