CREB5 Antibody / CRE-BPa

• Catalog Number: orb2636875
• Category: Antibodies
• Description: The ATF/CREB family consists of a series of transcription factors that function by binding to the cAMP responsive element (CRE) palindromic octanucleotide, TGACCTCA. The best characterized members of this gene family include CREB-1, CREB-2 (also designated ATF-4), CRE-BPa, LZIP (also designated CREB-3 and Luman), CREM-2, ATF-1, ATF-2, ATF-3, ATF-5, ATF-6 and ATF-7. These transcription factors share terminal leucine zipper dimerization and basic DNA binding domains and are highly variable in their N-termini. Although each of the ATF/ CREB proteins bind CREs in their homodimeric form, they can also bind as heterodimers, both within the ATF/CREB family and with members of the AP-1 transcription factor family. Protein kinase A- mediated CREB phosphorylation induces association with a nuclear protein designated CBP (CREB-binding protein), which may represent a CREB coactivator. CRE-BPa is a nuclear protein that binds DNA as a homodimer but can also form a heterodimer with ATF-2 or Jun.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: PCRP-CREB5-1G8
• Tested applications: FACS, IF, WB
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: Recombinant full-length human CREB5 protein was used as the immunogen for the CREB5 antibody.
• Antibody Type: Primary Antibody
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CREB5 antibody is available for research use only.
• UniProt ID: Q02930
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CREB5 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Immunofluorescent staining of PFA-fixed K562 cells stained with CREB5 antibody (green, clone PCRP-CREB5-1G8) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with CREB5 antibody (blue, clone PCRP-CREB5-1G8), and unstained cells (red).

FACS staining of PFA-fixed human K562 cells with CREB5 antibody (blue, clone PCRP-CREB5-1G8), and unstained cells (red).

Western blot testing of human HeLa cell lysate using CREB5 antibody (clone PCRP-CREB5-1G8). Predicted molecular weight: ~57 kDa (alpha), ~56 kDa (beta), ~53 kDa (gamma), ~41 kDa (delta).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CREB5 antibody (clone PCRP-CREB5-1G8). These results demonstrate the foremost specificity of the PCRP-CREB5-1G8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CREB5 antibody (clone PCRP-CREB5-1G8) as confirmation of integrity and purity.