CREB5 Antibody / CRE-BPa

• Catalog Number: orb1151622
• Category: Antibodies
• Description: Mouse monoclonal antibody to CREB5
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG2b, kappa
• Conjugation: Unconjugated
• Reactivity: Human
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Purity: Protein A/G affinity
• Immunogen: Recombinant full-length human CREB5 protein was used as the immunogen for the CREB5 antibody.
• UniProt ID: Q02930
• Tested applications: FACS, IF, WB
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml
• Application notes: Optimal dilution of the CREB5 antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: PCRP-CREB5-1G8
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Aliquot the CREB5 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.
• Hazard Information: This CREB5 antibody is available for research use only.
• Note: For research use only

Immunofluorescent staining of PFA-fixed K562 cells stained with CREB5 antibody (green, clone PCRP-CREB5-1G8) and phalloidin (red).

FACS staining of PFA-fixed human HeLa cells with CREB5 antibody (blue, clone PCRP-CREB5-1G8), and unstained cells (red).

FACS staining of PFA-fixed human K562 cells with CREB5 antibody (blue, clone PCRP-CREB5-1G8), and unstained cells (red).

Western blot testing of human HeLa cell lysate using CREB5 antibody (clone PCRP-CREB5-1G8). Predicted molecular weight: ~57 kDa (alpha), ~56 kDa (beta), ~53 kDa (gamma), ~41 kDa (delta).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CREB5 antibody (clone PCRP-CREB5-1G8). These results demonstrate the foremost specificity of the PCRP-CREB5-1G8 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CREB5 antibody (clone PCRP-CREB5-1G8) as confirmation of integrity and purity.