COPA Antibody

• Catalog Number: orb1248549
• Category: Antibodies
• Description: COPA Antibody
• Target: COPA
• Clonality: Polyclonal
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Porcine, Rat
• Form/Appearance: Liquid
• Concentration: 500 ug/mL
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Immunogen: The immunogen for this antibody is: CTRASNLENST
• UniProt ID: P53621
• MW: Approx 140kDa
• Tested applications: ELISA, WB
• Application notes: Peptide ELISA: antibody detection limit dilution 1:1000.Western Blot:Approx 140kDa band observed in Human Prostate lysates (calculated MW of 138kDa according to NP_001091868.1and NP_004362.2). Recommended concentration: 0.5-2ug/ml. An additional band of 52kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: COPA, coatomer protein complex, subunit alpha, FLJ
• Research Area: Signal Transduction
• Note: For research use only
• NCBI: NP_004362.2, NP_001091868.1

orb1248549 (0.5 ug/ml) staining of Human Prostate lysate (35 ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.