Complement C4d Antibody

SKU: orb1538195

Description

Complement C4d Antibody

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IHC, IHC-Fr, IHC-P
• Dilution Range: IHC, IHC-Fr (1:100 - 1:200), IHC-P (1:25 - 1:100)
• Reactivity: Human
• Application Notes:
  Further information: Immunohistochemistry: This was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working dilution for This was determined to be 1:50.

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Key Properties

• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Peptide derived from internal sequence of human C4.
• Target: Complement C4d
• Purification: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 20 mM Tris-HCl, pH 8.0, 20 mg/ml rAlbumin, 0.05% Sodium Azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Quick Database Links

Gene Symbol

Complement C4d