Complement C3d fragment Rabbit Polyclonal Antibody

• Catalog Number: orb156424
• Category: Antibodies
• Description: Complement C3d fragment Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Complement C3d fragment (1002-1100/1663aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 33/109/181 kDa
• Target: C3
• UniProt ID: P01024
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: Acylation stimulating protein; ASP; C3; C3a anaphy
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (Human brain glioma), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Complement C3d fragment) Polyclonal Antibody, Unconjugated (orb156424) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: 293T (Human) cell Lysate at 40 ug, Primary: Anti-Complement C3d fragment (orb156424) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-RabbitIgG at 1/20000 dilution, Predicted band size: 33'109'181kD, Observed band size: 33 kD.

Sample: Lane 1: Human HepG2 cell lysates, Lane 2: Human U87MG cell lysates, Lane 3: Human A431 cell lysates, Lane 4: Human HeLa cell lysates, Lane 5: Human A549 cell lysates, Primary: Anti-Complement C3d fragment (orb156424) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 33/109/187 kDa, Observed band size: 75 kDa.