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Catalog Number | orb867293 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to Collagen Type I |
Species/Host | Rabbit |
Tested applications | ELISA, IF, IHC-P, RIA, WB |
Immunogen | Purified collagen type I from human skin |
Concentration | app. 1 mg/ml |
Dilution range | (purified, lyophilized) IFA 1:80, IHC(P) 1:200 - 1:600, ELISA >1:200 (OD>500) |
Conjugation | Unconjugated |
Hazard Information | *These antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. |
UniProt ID | P08123, P02452 |
Storage | Storage: -20°C |
Buffer/Preservatives | affinity purified antibody lyophilized from phosphate buffered solution; no rAlbumin and preservative added! |
Note | For research use only |
Application notes | Positive Control: Human skin. Pre-Treatment: After de-waxing the tissue slices they are treated with 0.2% hyaluronidase (app. 300 U/mg e.g. Art. No. HYA02-50) in TBS 15 min at 37°C. Thereafter non-specific binding is blocked by blocking serum or 3% BSA in TBS. For peroxidase systems blocking with 1% peroxide solution in TBS for 30 min at RT is recommended. Incubation Time: IHC(P) 60 min at RT or 2-8°C over night |
Expiration Date | 12 months from date of receipt. |
IHC-P, WB | |
Bovine, Equine, Monkey, Sheep | |
Canine, Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
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