Collagen I/COL1A1 Antibody

• Catalog Number: orb107158
• Category: Antibodies
• Description: Collagen I/COL1A1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IF, IHC, WB
• Predicted Reactivity: Hamster
• Reactivity: Human, Mouse, Rat
• Isotype: Rabbit IgG
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of mouse Collagen I (1192-1207aa QPPQEKSQDGGRYYRA), identical to the related rat sequence, and different from the related human sequence by two amino acids.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Dilution range: Western blot, 0.1-0.5μg/ml, Human, Rat, Mouse Immunohistochemistry (Paraffin-embedded Section), 1-2μg/ml, Human, Mouse, Rat, By Heat Immunohistochemistry (Frozen Section), 0.5-1μg/ml, Human, Rat, Mouse Immunocytochemistry , 0.5-1μg/ml, Mouse, Human, Rat Immunofluorescence, 2μg/ml, Human
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 138032 MW
• UniProt ID: P11087
• Storage: Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
• Alternative names: Collagen alpha-1 (I) chain;Alpha-1 type I collagen
• Note: For research use only
• Application notes: WB: The detection limit for COL1A1 is approximately 0.5ng/lane under reducing conditions. Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections. Other applications have not been tested. Optimal dilutions should be determined by end users. . Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
• Expiration Date: 12 months from date of receipt.

IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.Collagen I/COL1A1 was detected in paraffin-embedded section of Human Intestinal Cancer tissues.

IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.Collagen I/COL1A1 was detected in paraffin-embedded section of mouse lung tissues.

IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.Collagen I/COL1A1 was detected in paraffin-embedded section of rat lung tissues.

IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.Collagen I/COL1A1 was detected in frozen section of Human Placenta Tissue.

IHC analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.Collagen I/COL1A1 was detected in immunocytochemical section of NIH3T3 Cell

IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.Collagen I/COL1A1 was detected in paraffin-embedded section of human mammary tissue tissues.

IF analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.Collagen I/COL1A1 was detected in paraffin-embedded section of human tonsil tissues.

Western blot analysis of Collagen I/COL1A1 using anti-Collagen I/COL1A1 antibody.