Collagen III Rabbit Polyclonal Antibody

• Catalog Number: orb10438
• Category: Antibodies
• Description: Collagen III Rabbit Polyclonal Antibody
• Target: COL3A1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Gallus
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Collagen alpha 1(III) chain (801-900/1466aa)
• UniProt ID: P02461
• RRID: AB_10935501
• MW: 117 kDa
• Tested applications: FC, ICC, WB
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test, ICC/IF=1:50-200
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: COL 3A1; COL3A1; Collagen alpha 1(III) chain; Coll
• Research Area: Cell Biology, Chondrogenesis, Collagen, Osteogenes
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: Hela Cell (Human) Lysate at 40 ug, Primary: Anti-Collagen III (orb10438) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117 kD, Observed band size: 130 kD.

Tissue/Cell: human colon cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated (orb10438) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Tissue/Cell: rat kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated (orb10438) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C.