Collagen II Rabbit Polyclonal Antibody

• Catalog Number: orb156419
• Category: Antibodies
• Description: Collagen II Rabbit Polyclonal Antibody
• Target: COL2A1
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Gallus, Mouse, Rabbit
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from rabbit Collagen II Chondrocalcin (1401-1487/1487aa)
• MW: 117/163 kDa
• Tested applications: WB
• Dilution range: WB=1:500-2000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Chondrocalcin; Collagen II alpha 1; COL2A1; COL2A1
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: HepG2 (Human) Cell Lysate at 30 ug, 293T (Human) Cell Lysate at 30 ug, Primary: Anti-Collagen II (orb156419) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 116 kD, Observed band size: 116 kD.

Sample: U2OS Cell (Human) Lysate at 40 ug, MDA-MB-231 Cell (Human) Lysate at 40 ug, Primary: Anti-Collagen II (orb156419) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 117/163 kD, Observed band size: 100 kD.