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Catalog Number | orb345344 |
---|---|
Category | Antibodies |
Description | Collagen I antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | DOT, ELISA, FC, FLISA, IF, IHC, IP, WB |
Reactivity | Bovine, Human, Mouse, Porcine, Rat |
Isotype | IgG |
Immunogen | Collagen Type I from human and bovine placenta |
Concentration | 1.00 mg/mL |
Dilution range | ELISA: 1:5,000 - 1:50,000, FLISA: 1:100, FC: User Optimized, IHC: 1:50 - 1:200, IF: User Optimized, IP: 1:100, WB: 1:1,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | COLLAGEN I Antibody has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested. |
Conjugation | Unconjugated |
UniProt ID | P02452 |
NCBI | NP_000079.2 |
Storage | Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | rabbit anti-collagen type I antibody, Collagen Of Read more... |
Note | For research use only |
Application notes | Anti-Collagen Type I has been tested by dot blot and IHC and are useful for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, immunoprecipitation, native (non-denaturing, non-dissociating) PAGE, immunohistochemistry, Immunofluorescence, FLOW, and western blotting for highly sensitive qualitative analysis. |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of Wistar rat hepatic stellate cells using COL1A1 antibody
IHC-P, WB | |
Bovine, Equine, Monkey, Sheep | |
Canine, Human, Mouse, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ICC, IF, IHC, WB | |
Hamster | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Porcine, Rat, Sheep | |
Rabbit | |
Polyclonal | |
Unconjugated |
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