COL1A1 antibody

• Catalog Number: orb345831
• Category: Antibodies
• Description: COL1A1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IHC, IP, WB
• Reactivity: Bovine, Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Collagen Type I from human and bovine placenta.
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:3,000 - 1:6,000, FC: 5µg/mL, IHC: 1:50 - 1:200, IP: 1:100, WB: 1:3,000 - 1:6,000
• Form/Appearance: Lyophilized
• Purity: This product has been prepared by immunoaffinity chromatography using immobilized antigens. Some class-specific anti-collagens may be specific for three-dimensional epitopes which may result in diminished reactivity with denatured collagen or formalin-fixed, paraffin embedded tissues. This antibody reacts with most mammalian Type I collagens and has expected cross-reactivity with Type III and negligible cross reactivity with Type II, IV, V or VI collagens. Non-specific cross-reaction of anti-collagen antibodies with other human serum proteins or non-collagen extracellular matrix proteins has not been tested.
• Conjugation: FITC
• UniProt ID: P02452
• NCBI: NP_000079.2
• Storage: Store vial at 4° C prior to restoration. Restore with 0.05 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of restoration.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-collagen type I antibody fluorescein c
• Note: For research use only
• Application notes: Anti-COLLAGEN Type I Fluorescein Conjugated Antibody is suitable for western blot, immunoprecipitation, Flow Cytometry, and immunohistochemistry. Researchers should determine optimal titers for applications that are not stated below.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Wistar rat hepatic stellate cells using COL1A1 antibody