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CNOT8 Antibody

Catalog Number: orb1263820

DispatchUsually dispatched within 5-10 working days
$ 880.00
Catalog Numberorb1263820
CategoryAntibodies
DescriptionCNOT8 Antibody
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsFC, IF, IHC-P, WB
Predicted ReactivityMouse
ReactivityHuman
IsotypeRabbit Ig
ImmunogenThis CNOT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 227-255 amino acids from the C-terminal region of human CNOT8.
Antibody TypePrimary Antibody
Concentrationbatch dependent
Form/AppearanceLiquid
ConjugationUnconjugated
MW34 kDa
TargetCNOT8
UniProt IDQ9UFF9
NCBIQ9UFF9
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied in PBS with 0.09% (W/V) sodium azide.
Alternative namesCCR4-NOT transcription complex subunit 8, CAF1-lik
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NoteFor research use only
Application notesFor WB starting dilution is: 1:1000For IF starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50For FACS starting dilution is: 1:10~50
Expiration Date12 months from date of receipt.
CNOT8 Antibody

Western blot analysis in U251 cell line lysates (35 ug/lane).

CNOT8 Antibody

Fluorescent confocal image of Hela cell stained with CNOT8 Antibody.Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with CNOT8 primary antibody (1:25). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). CNOT8 immunoreactivity is localized to Cytoplasm significantly and Nucleus weakly.

CNOT8 Antibody

Formalin-fixed and paraffin-embedded human colon carcinoma with CNOT8 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

CNOT8 Antibody

Flow cytometric analysis of CEM cells using CNOT8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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