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Catalog Number | orb1274242 |
---|---|
Category | Antibodies |
Description | CNN1 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | CNN1/1408R |
Tested applications | FC, IF, IHC-P |
Reactivity | Human, Rat |
Isotype | IgG, k |
Immunogen | Recombinant human protein was used as the immunogen for the recombinant Calponin antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1ug/10^6 cellsImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CNN1 |
UniProt ID | P51911 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | CNN1, Basic calponin, Calponin, Calponin-1, Calpon Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1ug/10^6 cellsImmunofluorescence: 0.5-1 ug/mlImmunohistochemistry (FFPE): 1-2 ug/ml for 30 min at RTPrediluted IHC only format: incubate for 30 min at RT (1)Optimal dilution of the recombinant Calponin antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
IHC testing of FFPE human uterus stained with recombinant Calponin antibody (clone CNN1/1408R). HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
IHC testing of FFPE rat uterus stained with recombinant Calponin antibody (clone CNN1/1408R). HIER: steam sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min.
FC, IHC-P | |
Bovine, Canine, Equine, Human, Mouse, Porcine | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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