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Catalog Number | orb1252576 |
---|---|
Category | Antibodies |
Description | CNN1 Antibody |
Species/Host | Mouse |
Clonality | Monoclonal |
Clone Number | CNN1/832 |
Tested applications | FC, IF, IHC-P |
Reactivity | Human, Rat |
Isotype | IgG1, kappa |
Immunogen | Recombinant human protein was used as the immunogen for the anti-Calponin antibody. |
Concentration | 0.2 mg/mL |
Dilution range | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the anti-Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CNN1 |
UniProt ID | P51911 |
Storage | Aliquot and Store at 2-8°C. Avoid freez-thaw cycles. |
Buffer/Preservatives | PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide |
Alternative names | CNN1, Basic calponin, Calponin, Calponin-1, Calpon Read more... |
Note | For research use only |
Application notes | Flow Cytometry: 0.5-1 ug/million cells in 0.1mlImmunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the anti-Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min. |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed, paraffin-embedded human breast carcinoma stained with anti-Calponin antibody (CNN1/832).
Formalin-fixed, paraffin-embedded human uterus stained with anti-Calponin antibody (CNN1/832).
Formalin-fixed, paraffin-embedded rat uterus stained with anti-Calponin antibody (CNN1/832).
FC, IHC-P | |
Bovine, Canine, Equine, Human, Mouse, Porcine | |
Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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