Clusterin Antibody / Apolipoprotein J / APO-J

• Catalog Number: orb2636643
• Category: Antibodies
• Description: Clusterin, also designated complement lysis inhibitor (CLI), apolipoprotein J (APOJ), sulfated glycoprotein 2 (SGP2), SP40 and testosterone-repressed prostate message 2 (TRPM2), is a secretory, heterodimeric glycoprotein that influences immune regulation, cell adhesion, transformation, lipid transportation, tissue remodeling, membrane recycling and cell-cell interactions. Clusterin is synthesized as a 449 amino acid polypeptide that is post-translationally cleaved at an internal bond between Arg 227 and Ser 228. The beta subunit corresponds to residues 23-227. The alpha subunit corresponds to residues 228-449. Overexpression of Clusterin appears to be more common in late stages of mammary tumor progression. Clusterin markedly influences Amyloid structure and neuritic toxicity in vivo and may influence Alzheimer s pathogenesis.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: CLU/4727
• Tested applications: IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG2c, kappa
• Immunogen: A portion of amino acids 185-287 was used as the immunogen for the CLU antibody.
• Antibody Type: Primary Antibody
• Dilution range: Western blot: 2-4ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml
• Purity: Protein A/G affinity
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This CLU antibody is available for research use only.
• UniProt ID: P10909
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the CLU antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human adrenal gland with CLU antibody (clone CLU/4727). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot analysis of human testis tissue lysate using CLU antibody (clone CLU/4727). Predicted molecular weight: 75-80 kDa (heterodimer precursor), 36-39 kDa (alpha subunit), 34-36 kDa (beta subunit).

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using CLU antibody (clone CLU/4727). These results demonstrate the foremost specificity of the CLU/4727 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.

SDS-PAGE analysis of purified, BSA-free CLU antibody (clone CLU/4727) as confirmation of integrity and purity.