CLU antibody

• Catalog Number: orb43569
• Category: Antibodies
• Description: Mouse monoclonal antibody to CLU
• Clonality: Monoclonal
• Clone Number: Hs-3
• Tested applications: ELISA, ICC, IHC-P, WB
• Reactivity: Human
• Isotype: Mouse IgG1
• Immunogen: Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
• Concentration: 1 mg/ml
• Dilution range: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: Sertoli cells. Western blotting: Recommended dilution: 1 μg/ml; positive material: seminal plasma.
• Purity: Purified by protein-A affinity chromatography.
• Conjugation: Unconjugated
• Target: Clusterin
• Entrez: 1191
• UniProt ID: P10909
• Storage: Store at 2-8°C. Do not freeze.
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Alternative names: anti Clusterin antibody, anti CLU antibody, anti A
• Note: For research use only
• Application notes: Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: Sertoli cells. Western blotting: Recommended dilution: 1 μg/ml; positive material: seminal plasma.
• Expiration Date: 12 months from date of receipt.

Western blotting analysis of human clusterin using mouse monoclonal antibody Hs-3 on lysates of MCF-7 cell line and MOLT-4 cell line (clusterin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-clusterin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Clusterin was detected around 40 kDa.