CLEC2D Rabbit Polyclonal Antibody (FITC)

• Catalog Number: orb8736
• Category: Antibodies
• Description: CLEC2D Rabbit Polyclonal Antibody (FITC)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Canine, Equine, Human, Porcine, Rabbit, Rat
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CLEC2D (101-207/207aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg /test
• Conjugation: FITC
• Target: CLEC2D
• RRID: AB_10925559
• Storage: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: C-type lectin domain family 2 member D; C type lec
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control (blue line): Raw264.7 (blue). Primary Antibody (red line): Rabbit Anti-CLEC2D antibody (orb8736) in Overexpression hCLEC2D Raw264.7, dilution: 1 µg/10^6 cells, Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed. Tongji University/Professor Jia's laboratory.

Blank control: Mouse spleen. Primary Antibody (green line): Rabbit Anti-CLEC2D antibody (orb8736), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. Acquisition of 20000 events was performed.