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Catalog Number | orb1246737 |
---|---|
Category | Antibodies |
Description | CLEC16A Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, FC, IF |
Predicted Reactivity | Mouse, Rat |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-SLENQDKGGERP |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 70kDa band in Rat Testis lysates after 0.5ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for this band, given the calculated size of 115kDa according to Rat XP_213209.4.Immunofluorescence: Strong expression of the protein seen in the nuclei and weak expression seen in the cytoplasm of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CLEC16A |
UniProt ID | Q2KHT3 |
NCBI | NP_056041.1, NP_001230332.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | CLEC16A, C-type lectin domain family 16, member A, Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 70kDa band in Rat Testis lysates after 0.5ug/ml antibody staining. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation 1 hour at room temperature. Please note that we currently cannot find an explanation in the literature for this band, given the calculated size of 115kDa according to Rat XP_213209.4.Immunofluorescence: Strong expression of the protein seen in the nuclei and weak expression seen in the cytoplasm of A549 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of A549 cells. Recommended concentration: 10ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1246737 Immunofluorescence analysis of paraformaldehyde fixed A549 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (2 ug/ml), showing strong nuclear and weak cytoplasmic staining.
orb1246737 Flow cytometric analysis of paraformaldehyde fixed A549 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line).
IHC-P, WB | |
Bovine, Mouse, Porcine, Rat | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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