Claudin 5 Rabbit Polyclonal Antibody

• Catalog Number: orb182867
• Category: Antibodies
• Description: Claudin 5 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat, Sheep
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Claudin 5 (29-81/218aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 23 kDa
• Target: CLDN5
• UniProt ID: O00501
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: AWAL; BEC 1; BEC1; Claudin 5(transmembrane protein
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Maysam Havasi Mehr 1, Shahein Momenabadi 2, Ali Vakili 3, Abbas Pakdel 4, Abbas Ali Vafaei 5, Abedin Vakili Neuroprotective effects of Daphnetin on hippocampal neurons and blood-brain barrier integrity in a mouse model of cerebral ischemia Brain Res Bull ., (2024)

PubMed: 39447767

Applications

IF

Reactivity

Mouse

Protein: lung (mouse) lysates at 45 ug, Primary: Anti-Claudin 5 (orb182867) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000, ECL excitated the fluorescence, Predicted band size: 23 kD, Observed band size: 23kD.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (orb182867), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Claudin 5) Polyclonal Antibody, Unconjugated (orb182867) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.