Claudin 5 Rabbit Polyclonal Antibody

• Catalog Number: orb182867
• Category: Antibodies
• Description: Claudin 5 Rabbit Polyclonal Antibody
• Target: CLDN5
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Equine, Porcine, Rabbit, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Purification: Affinity purified by Protein A
• Immunogen: KLH conjugated synthetic peptide derived from human Claudin 5 (29-81/218aa)
• UniProt ID: O00501
• MW: 23 kDa
• Tested applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=2ug/Test
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: AWAL; BEC 1; BEC1; Claudin 5(transmembrane protein
• Research Area: Signal Transduction
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Maysam Havasi Mehr 1, Shahein Momenabadi 2, Ali Vakili 3, Abbas Pakdel 4, Abbas Ali Vafaei 5, Abedin Vakili Neuroprotective effects of Daphnetin on hippocampal neurons and blood-brain barrier integrity in a mouse model of cerebral ischemia Brain Res Bull ., (2024)

PubMed: 39447767

Applications

IF

Reactivity

Mouse

Yuanyuan Bai 1 2, Haiyan Wu 1 3, Xu Wang 4, Yang Guo 1 2, Bingqing Gong 1 2, Beibei Dong 2, Yonghao Yu A mitochondrial-derived peptide MOTS-c contributes to the protective effect against brain injury associated with LPS-induced sepsis by strengthening the blood-brain barrier's ultrastructure Int J Neurosci, (2025)

PubMed: 40753494

Applications

WB

Reactivity

Mouse

Jessica J Avilez-Avilez 1 2, Jesús Enrique García-Aviles # 1 3, Ricardo Jair Ramírez-Carreto # 4 5, Verónica Salas-Venegas 4 6, Mara A Guzmán-Ruiz 3, Fernanda Medina-Flores 7, Mina Königsberg 8, Anahí Chavarría 9, Beatriz Gómez-González Progressive Blood-Brain Barrier Disruption in Sleep-Restricted Young Mice: Cellular Senescence and Neuroinflammation Crosstalk Neurochem Res ., (2025)

PubMed: 40824389

Applications

WB

Reactivity

Mouse

Maydelín Espadín a b , Miguel Cervantes c , Angélica Coyoy-Salgado d , Claudia Espinosa-Garcia e , Beatriz Gómez-González b , Gabriela Moralí Progesterone restores blood-brain barrier and memory after chronic cerebral hypoperfusion in male rats Brain Behavior and Immunity Integrative, (2025)

PubMed:

Protein: lung (mouse) lysates at 45 ug, Primary: Anti-Claudin 5 (orb182867) at 1:300, Secondary: HRP conjugated Goat-Anti-Rabbit IgG at 1:5000, ECL excitated the fluorescence, Predicted band size: 23 kD, Observed band size: 23kD.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-Claudin 5 antibody (orb182867), dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-FITC, dilution: 0.5 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Paraformaldehyde-fixed, paraffin embedded (Human kidney), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Claudin 5) Polyclonal Antibody, Unconjugated (orb182867) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.