CIRBP antibody

• Catalog Number: orb20642
• Category: Antibodies
• Description: Goat polyclonal to CIRBP
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, WB
• Reactivity: Bovine, Canine, Human, Mouse, Rat
• Dilution range: ELISA: 1:16000, WB: 0.05-0.2 μg/ml
• Conjugation: Unconjugated
• MW: 18.6
• Target: CIRBP (aa 81-91)
• Entrez: 1153
• Protein Sequence: QAGKSSDNRSR
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti CIRBP antibody, anti cold inducible RNA bindi
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 18kDa band observed in Mouse and Rat Testis lysates (calculated MW of 18.6kDa according to Mouse NP_031731.1 and Rat NP_112409.2). Recommended concentration: 0.05-0.2 μg/ml. An additional band of unknown identity was also consistently observed at 23kDa. This band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any such results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported
• Expiration Date: 12 months from date of receipt.

orb20642 (0.05ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.