CIAPIN1 Antibody

• Catalog Number: orb1257083
• Category: Antibodies
• Description: CIAPIN1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-312 of human CIAPIN1 (NP_064709.2).
• Antibody Type: Primary Antibody
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 39kDa
• Target: CIAPIN1
• UniProt ID: Q6FI81
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: Anamorsin {ECO:0000255|HAMAP-Rule:MF_03115}, Cytok
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:100
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using CIAPIN1 antibody (orb1257083) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Immunohistochemistry of paraffin-embedded human liver damage using CIAPIN1 Antibody (orb1257083) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human gastric cancer using CIAPIN1 Antibody (orb1257083) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using CIAPIN1 Antibody (orb1257083) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of U2OS cells using CIAPIN1 antibody (orb1257083). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells, using 3 ug CIAPIN1 antibody (orb1257083). Western blot was performed from the immunoprecipitate using CIAPIN1 antibody (orb1257083) at a dilution of 1:1000.