CHUK Antibody

• Catalog Number: orb1258311
• Category: Antibodies
• Description: CHUK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 650 to the C-terminus of human IKK alpha (NP_001269.3).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 94kDa
• Target: CHUK
• UniProt ID: O15111
• Storage: Store at -20°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Alternative names: IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKB
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200IP: 1:50 - 1:200
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using IKK alpha antibody (orb1258311) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunohistochemistry of paraffin-embedded rat brain using IKK alpha antibody (orb1258311) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse kidney using IKK alpha antibody (orb1258311) at dilution of 1:100 (40x lens).

Immunofluorescence analysis of A549 cells using IKK alpha antibody (orb1258311). Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 200 ug extracts of 293T cells using 3 ug IKK alpha antibody (orb1258311). Western blot was performed from the immunoprecipitate using IKK alpha antibody (orb1258311) at a dilution of 1:1000.