CHUK Antibody

• Catalog Number: orb1239457
• Category: Antibodies
• Description: CHUK Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Bovine, Mouse
• Reactivity: Human
• Isotype: IgG
• Immunogen: IKK alpha antibody was raised against a 19 amino acid peptide near the carboxy terminus of human IKK alpha.The immunogen is located within the last 50 amino acids of IKK alpha.
• Concentration: 1 mg/ml
• Dilution range: IKK alpha can be used for detection of IKK alpha by Western blot at 1 μg/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 85 kDa
• Target: CHUK
• UniProt ID: O15111
• NCBI: AF009225
• Storage: IKK alpha antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: IKK alpha Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: IKK alpha Antibody: IKK1, IKKA, IKBKA, TCF16, NFKB
• Note: For research use only
• Application notes: IKK alpha can be used for detection of IKK alpha by Western blot at 1 μg/mL. An 85 kDa band should be detected. Antibody can also be used for immunocytochemistry starting at 1 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IKK alpha in HeLa whole cell lysate with IKK alpha antibody at 1:1000 dilution.

Immunocytochemistry of IKK alpha in Jurkat cells with IKK alpha antibody at 1 µg/mL.

Immunofluorescence of IKK alpha in Jurkat cells with IKK alpha antibody at 10 µg/mL.

Immunofluorescence of IKKa in Jurkat cells with IKK alpha antibody at 20 µg/mL. Green: IKK alpha Antibody (orb1239457) Blue: DAPI staining.

Immunocytochemistry of IKKa in Jurkat cells with IKK alpha antibody at 2 µg/mL.