CHST7 Rabbit Polyclonal Antibody

SKU: orb182627

Description

CHST7 Rabbit Polyclonal Antibody

Research Area

Cell Biology, Energy Metabolism, Glycosaminoglycans (GAGs)

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Mouse
• Predicted Reactivity: Bovine, Canine, Human, Porcine, Rabbit, Rat, Sheep

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CHST7 (101-200/486aa)
• Target: CHST7
• Molecular Weight: 54 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

6 O sulfotransferase 5; C6ST 2; C6ST2; Carbohydrate sulfotransferase 7; Chondroitin 6 sulfotransferase 2; CHST 7; CHST7_HUMAN; CHST7 carbohydrate(N-acetylglucosamine 6-O) sulfotransferase 7; Galactose/N acetylglucosamine/N acetylglucosamine 6 O sulfotransferase 5; GlcNAc6ST 4; Gn6st-4; GST 5; N acetylglucosamine 6 O sulfotransferase 1.

Paraformaldehyde-fixed, paraffin embedded (Mouse brain), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CHST7) Polyclonal Antibody, Unconjugated (orb182627) at 1:400 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Kidney (Mouse) Lysate at 40 ug Cerebrum (Mouse) Lysate at 40 ug Primary: Anti-CHST7 (orb182627) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 54 kD Observed band size: 54 kD.

Sample: Ovary (Mouse) Lysate at 40 ug, Primary: Anti-CHST7 (orb182627) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 54 kD, Observed band size: 54 kD.

Quick Database Links

Gene Symbol

CHST7

UniProt

Q9NS84