CHST1 Rabbit Polyclonal Antibody

SKU: orb157743

Description

CHST1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC
• Dilution range: Flow-Cyt=1μg/Test
• Reactivity: Rat
• Predicted Reactivity: Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CHST1 (251-350/411aa)
• Target: CHST1
• Molecular Weight: 45 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

Carbohydrate chondroitin 6 keratan sulfotransferase 1; Carbohydrate chondroitin 6 keratan sulfotransferase; Carbohydrate keratan sulfate Gal 6 sulfotransferase; Carbohydrate keratan sulfate Gal 6 sulfotransferase 1; Carbohydrate sulfotransferase 1; CHST1; EC 2.8.2.21; Galactose N acetylglucosamine N acetylglucosamine 6 O sulfotransferase 1; GST 1 antibody Keratan sulfate Gal 6 sulfotransferase; KS6ST; KSGAL6ST; KSST.

Blank control (blue): RSC96 cells (fixed with 2% paraformaldehyde (10 min), then permeabilized with 90% ice-cold methanol for 30 min on ice). Primary Antibody: Rabbit Anti-Keratan Sulfate antibody (orb157743), Dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), Dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Cerebrum (Mouse) Lysate at 40 ug, Primary: Anti-Keratan Sulfate (orb157743) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 45 kD, Observed band size: 45 kD.

Quick Database Links

Gene Symbol

CHST1

UniProt

O43916