CHSS2 Rabbit Polyclonal Antibody

• Catalog Number: orb500747
• Category: Antibodies
• Description: CHSS2 Rabbit Polyclonal Antibody
• Target: CHPF
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat, Sheep
• Form/Appearance: Liquid
• Concentration: 1mg/ml
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Immunogen: KLH conjugated synthetic peptide derived from human CHSS2 (51-150/775aa)
• UniProt ID: Q8IZ52
• MW: 85 kDa
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Chondroitin glucuronyltransferase II; Chondroitin
• Note: For research use only

Sample: Mcf-7 (human) Cell Lysate at 40 ug, Primary: Anti-CHSS2 (orb500747) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.

Sample: Siha (human) Cell Lysate at 40 ug, Primary: Anti-CHSS2 (orb500747) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 85 kD, Observed band size: 85 kD.

Tissue/Cell: human colon carcinoma, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CHSS2 Polyclonal Antibody, Unconjugated (orb500747) 1:400, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.