Chromogranin A Antibody / CHGA

• Catalog Number: orb699498
• Category: Antibodies
• Description: Chromogranin A (CGA) is an acidic protein which is reported to be widely expressed in neural tissues and in secretory granules of human endocrine cells e.g. parathyroid gland, adrenal medulla, anterior pituitary gland, islet cells of the pancreas and C cells of the thyroid. GA is found in the large majority of epithelial NE tumors: pituitary adenomas, parathyroid and pancreatic islet cell tumors, medullary thyroid carcinoma, and carcinoid tumors. Together with synaptophysin, CGA is the most important marker for the identification of NE and neuronal tumors and NE/neuronal differentiation in other tumors.
• Clonality: Recombinant
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Immunogen: A portion of amino acids 400-600 from the human protein was used as the immunogen for the recombinant Chromogranin A antibody.
• UniProt ID: P10645
• Tested applications: IHC-P
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Application notes: Optimal dilution of the recombinant Chromogranin A antibody should be determined by the researcher.
• Antibody Type: Primary Antibody
• Clone Number: CHGA/4544R
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Hazard Information: This recombinant Chromogranin A antibody is available for research use only.
• Note: For research use only

IHC staining of FFPE human pancreas with recombinant Chromogranin A antibody (clone CHGA/4544R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human pancreas with recombinant Chromogranin A antibody (clone CHGA/4544R). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.