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CHRNB4 Antibody

SKU: orb1246565

Description

CHRNB4 Antibody

Research Area

Neuroscience

Images & Validation

Tested ApplicationsELISA, IHC
ReactivityHuman
Predicted ReactivityBovine
Application Notes
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Preliminary experiments gave an approx 40kDa band in human testis lysates after 0.2µg/mL antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 56.4kDa according to NP_000741.1. The 40kDa band was successfully blocked by incubation with the immunizing peptide.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 2.5µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-DYRLTWNSSRYEGVN
TargetCHRNB4
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

CHRNB4 , cholinergic receptor, nicotinicN: beta 4 , Cholinergic receptor, neuronal nicotinic, beta polypeptide-4, cholinergic receptor, nicotinic, beta polypeptide 4, neuronal nicotinic receptor beta 4 subunit

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CHRNB4 Antibody

orb1246565 (2.5 ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

Documents Download

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Protocol Information

IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

CHRNB4 Antibody (orb1246565)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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