CHRNA7 Rabbit Polyclonal Antibody

• Catalog Number: orb312180
• Category: Antibodies
• Description: CHRNA7 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CHRNA7 (441-502/502aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 55 kDa
• Target: CHRNA7
• UniProt ID: Q8IUZ4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: CHRFAM7A; ACHA7_HUMAN; cholinergic receptor, nicot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (orb312180) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Adrenal glands (Rat) Lysate at 40 ug, Lane 4: Adrenal glands (Mouse) Lysate at 40 ug, Lane 5: Placenta (Mouse) Lysate at 40 ug, Lane 6: Lung (Mouse) Lysate at 40 ug, Lane 7: Testis (Rat) Lysate at 40 ug, Lane 8: HepG2 (Human) Cell Lysate at 30 ug, Lane 9: SH-SY5Y (Human) Cell Lysate at 30 ug, Primary: Anti-CHRNA7 (orb312180) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56'50 kD, Observed band size: 48 kD.

Sample: Lane 1: Human SH-SY5Y cell Lysates, Lane 2: Human U-87 MG cell Lysates, Lane 3: Human U251 cell Lysates, Primary: Anti-CHRNA7 (orb312180) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kDa, Observed band size: 55kDa.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated (orb312180) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.