CHRNA7 Rabbit Polyclonal Antibody

SKU: orb312180

Description

CHRNA7 Rabbit Polyclonal Antibody

Research Area

Hypoxia, Nach Receptors, Neuroscience

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Gallus

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CHRNA7 (441-502/502aa)
• Target: CHRNA7
• Molecular Weight: 55 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CHRNA7-2; NACHRA7; nAChR7; Acra7; alpha7; nAchR; BTX; NARAD; ACHA7_CHICK; CHRNA7; Nicotinic acetylcholine receptor subunit alpha-7; ACHA7_BOVIN; ACHA7_HUMAN; Q8IUZ4_HUMAN; ACHA7_MOUSE; ACHA7_RAT;

Paraformaldehyde-fixed, paraffin embedded (rat adrenal gland), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (CHRNA7) Polyclonal Antibody, Unconjugated (orb312180) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Cerebrum (Rat) Lysate at 40 ug, Lane 2: Cerebrum (Mouse) Lysate at 40 ug, Lane 3: Adrenal glands (Rat) Lysate at 40 ug, Lane 4: Adrenal glands (Mouse) Lysate at 40 ug, Lane 5: Placenta (Mouse) Lysate at 40 ug, Lane 6: Lung (Mouse) Lysate at 40 ug, Lane 7: Testis (Rat) Lysate at 40 ug, Lane 8: HepG2 (Human) Cell Lysate at 30 ug, Lane 9: SH-SY5Y (Human) Cell Lysate at 30 ug, Primary: Anti-CHRNA7 (orb312180) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 56'50 kD, Observed band size: 48 kD.

Sample: Lane 1: Human SH-SY5Y cell Lysates, Lane 2: Human U-87 MG cell Lysates, Lane 3: Human U251 cell Lysates, Primary: Anti-CHRNA7 (orb312180) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 55kDa, Observed band size: 55kDa.

Tissue/Cell: human kidney tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-CHRNA7 Polyclonal Antibody, Unconjugated (orb312180) 1:200, overnight at 4°C, The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugated (orb868589) used at 1:200 dilution for 40 minutes at 37°C. DAPI (5 ug/ml, blue) was used to stain the cell nuclei.

Quick Database Links

Gene Symbol

CHRNA7

UniProt

P36544

Raheleh Baradaran et al. The distribution map of α4 and α7 subunits of nAch receptors in the olfactory bulb of male rat neonates born to STZ-induced hyperglycemia model mothers Neurosci Lett ., (2026)

PubMed: 41558592

Applications

IHC

Reactivity

Rat