CHRM4 Rabbit Polyclonal Antibody

• Catalog Number: orb156359
• Category: Antibodies
• Description: CHRM4 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human CHRM4/mAChR M4 (1-31/479aa)
• Antibody Type: Primary Antibody
• Concentration: 1mg/ml
• Dilution range: Flow-Cyt=1μg/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 53 kDa
• Target: CHRM4
• UniProt ID: P08173
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: mAChR M4; Muscarinic Acetylcholine Receptor M4; Ch
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Black line: Positive blank control A431), Negative blank control (Huvec), Green line: Primary Antibody (Rabbit Anti-CHRM4 antibody (orb156359)), Orange line: Isotype Control Antibody (Rabbit IgG). Blue line: Secondary Antibody (Goat anti-rabbit IgG-AF488), A431 (Positive) and HUVEC Negative control) cells (black) were incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with CHRM4 Antibody (orb156359) at 1:50 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2% BSA in PBS, followed by secondary antibody (blue) incubation for 40 min at room temperature. Acquisitions of 20000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

Blank control (blue line): A431 cells (fixed with 70% ice-cold methanol overnight at 4°C). Primary Antibody (green line): Rabbit Anti-CHRM4/PE-CY7 Conjugated antibody, Dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG-PE-CY7.

Blank control: K562. Primary Antibody (green line): Rabbit Anti-CHRM4 antibody (orb156359), Dilution: 2 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, Dilution: 1 µg/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Human U-2 OS cell lysates, Lane 2: Human THP-1 cell lysates, Lane 3: Human MCF-7 cell lysates, Lane 4: Human A549 cell lysates, Primary: Anti-CHRM4 (orb156359) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 55 kD.