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Catalog Number | orb1249604 |
---|---|
Category | Antibodies |
Description | CHN2 Antibody |
Species/Host | Goat |
Clonality | Polyclonal |
Tested applications | ELISA, IHC |
Predicted Reactivity | Bovine, Canine, Mouse |
Reactivity | Human |
Immunogen | The immunogen for this antibody is: C-QILIENEDVLF |
Concentration | 500 ug/mL |
Dilution range | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Brain (Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Cortex shows staining of neuronal bodies. Recommended concentration, 3-6ug/ml. |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
Target | CHN2 |
UniProt ID | P52757 |
NCBI | NP_001035025.1, NP_004058.1 |
Storage | Aliquot and store at -20°C. Minimize freezing and thawing. |
Buffer/Preservatives | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
Alternative names | CHN2, chimerin (chimaerin) 2, ARHGAP3, RHOGAP3, Rh Read more... |
Note | For research use only |
Application notes | Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Preliminary experiments in Human Brain (Cerebellum and Frontal Cortex) lysates gave no specific signal but low background (at antibody concentration up to 1ug/ml). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?Immunohistochemistry:In paraffin embedded Human Cortex shows staining of neuronal bodies. Recommended concentration, 3-6ug/ml. |
Expiration Date | 12 months from date of receipt. |
orb1249604 (3.8 ug/ml) staining of paraffin embedded Human Cortex. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
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