CHK1 Antibody

SKU: orb345527

Description

CHK1 antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:2,000 - 1:10,000, IP: 1:100, WB: 1:200 - 1:2,000
• Reactivity: Yeast
• Application Notes:
  This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 40-60 kDa in size corresponding to CHK1 by western blotting in the appropriate cell lysate or extract.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 305-330 of S. cerevisiae CHK1.
• Target: CHK1
• Purity: This affinity purified antibody is directed against yeast CHK1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Reactivity occurs against yeast CHK1 protein. A BLAST analysis was used to suggest little to no cross reactivity with this protein from other sources.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 0.73 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

rabbit anti-CHK1 Antibody, CHK-1, CHK 1, Serine/threonine-protein kinase CHK1, Checkpoint kinase 1

Quick Database Links

Gene Symbol

CHK1

UniProt

P38147

RefSeq:6319751